| 摘要: |
| 目的 建立基于高效液相色谱技术的双酚A分析方法，用于快速准确检测襄阳市售5种PET瓶装饮用水塑料包装中的双酚A含量及其在饮用水中的迁移量。方法 样品经前处理后，采用高效液相色谱-紫外检测器方法分离检测，选择ODS-C18色谱柱（4.6 mm×150.0 mm，5 μm），流动相为甲醇-乙酸铵溶液（体积比为50∶50），柱温为30 ℃，流速为1 mL/min，检测波长为280 nm。结果 双酚A的检出限为0.004 μg/mL，在0.02 μg/mL质量浓度条件下的相对标准偏差为4.32%，在线性浓度为0.01~200 μg/mL内的检测信号与双酚A浓度具有良好的线性相关性（r2>0.9999），方法分析性能满足国标限量要求。襄阳市售主要品牌PET瓶装饮用水包装中虽均含有一定量的双酚A，但饮用水中未检出双酚A。PET包装中双酚A在0.4，4，40 μg/g等3个加标水平的回收率为70.30%~113.00%，饮用水中双酚A在0.1，1，10 μg/g等3个加标水平的回收率为90.00%~119.00%，加标回收结果良好。结论 此方法简便、准确、高效，适用于PET瓶装饮用水包装中双酚A含量及迁移量的分析。 |
| 关键词: 高效液相色谱法 饮用水 塑料包装 双酚A 迁移量 |
| DOI：10.19554/j.cnki.1001-3563.2019.23.011 |
| 分类号:TS206.4 |
| 基金项目:湖北省自然科学基金青年项目（2018CFB316）；湖北省教育厅中青年人才项目（Q20182602）；湖北省高等学校实验室研究项目（HBSY2018-33）；湖北文理学院科研基金项目（2017kypy006，2020170329，xk2018007） |
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| Determination of Bisphenol A and Its Migration in PET Bottled Drinking Water by High Performance Liquid Chromatography |
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WANG Jie, SHANG Jun-qi, SHI Juan, SUN Peng-wei, LI Huan-huan, FAN Wen-ying
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School of Food Science and Technology, Hubei University of Arts and Science, Xiangyang 441053, China
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| Abstract: |
| The work aims to establish a bisphenol A analysis method based on the high performance liquid chroma-tography to rapidly and accurately determine the bisphenol A content and its migration in 5 kinds of plastic packaging for PET bottled drinking water sold in Xiangyang. The pretreated sample was detected by high performance liquid chroma-tography-ultraviolet detection (HPLC-UV) with a column of ODS-C18 (4.6 mm×150.0 mm, 5 μm) at 30 ℃. The mobile phase was methanol-ammonium acetate (V∶V, 50∶50), and the flow rate was 1 mL/min. The detection wavelength was 280 nm. The limit of detection (LOD) of bisphenol A was 0.004 μg/mL, and the relative standard deviation (RSD) was 4.32% at the concentration of 0.02 μg/mL. When the linear concentration was within 0.01~200 μg/mL, the detection signal had a good linear correlation (r2＞0.9999) with bisphenol A concentration. The analysis capability of the method met the limited demand of national standard. Certain amount of Bisphenol A was found in the packaging of PET bottled drinking water of major brands sold in Xiangyang, while no bisphenol A was detected in drinking water. The recoveries of bisphenol A in PET packaging at the spiked concentration of 0.4, 4 and 40 μg/g were in the range of 70.30%~113.00%, and the recoveries of bisphenol A drinking water at the spiked concentration of 0.1, 1 and 10 μg/g were 90.00%~119.00%. The proposed method is simple, accurate and efficient, which is suitable for the analysis of bisphenol A content and its migration in the packaging of PET bottled drinking water. |
| Key words: high performance liquid chromatography drinking water plastic packaging bisphenol A migration |
