| 摘要: |
| 目的 以鸡蛋壳膜为原材料，采用碱盐法提取硫酸软骨素。方法 以多糖提取率为指标研究提取时间、提取温度、碱盐比和液料比对试验的影响。在单因素实验基础上，用响应曲面法分析优化试验的各种条件。结果 得到了提取蛋壳膜硫酸软骨素的最佳工艺参数，提取时间为8 h，提取温度为65 ℃，碱盐比为6∶1，液料比为15∶1。在此条件下，硫酸软骨素的提取率为74.0%。结论 通过响应曲面设计试验及验证试验证明该最佳反应条件具有一定的可靠性与可行性。 |
| 关键词: 硫酸软骨素 鸡蛋壳膜 提取 响应曲面 多糖 |
| DOI：10.19554/j.cnki.1001-3563.2019.17.008 |
| 分类号:TQ460 |
| 基金项目:黑龙江省教育厅项目（12541199）；黑龙江省自然科学基金面上项目（C2018036）；2018年度哈尔滨商业大学校级一般项目（18XN080） |
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| Use of Response Surface Analysis for Optimization of Extraction Process of Chondroitin Sulfate from Egg Shell Membrane |
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LIU Tao1, ZHANG Tie-peng1, LUAN Xin-yue2, LIU He1, LIU Ning1
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1.College of Food Engineering, Harbin University of Commerce, Harbin 150076, China;2.Taizhou Patent Strategy Promotion and Service Center Co., Ltd., Taizhou 225300, China
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| Abstract: |
| The work aims to extract chondroitin sulfate in the alkali-salt method with the egg shell membrane as raw material. The polysaccharide extraction rate was used as index to study the effects of extraction time, extraction temperature, alkali-salt ratio and liquid-solid ratio on the test. On the basis of single factor test, various conditions of test were analyzed and optimized in the response surface method. The experiment results showed that, the best process parameters for the extraction of egg shell membrane were as follows: extraction time of 8 h, extraction temperature of 65 ℃, alkali-salt ratio of 6∶1, and liquid-to-solid ratio of 15∶1. Under these conditions, the extraction rate of chondroitin sulfate was 74.0%. The response surface design test and verification test show that the proposed optimal reaction condition is reliable and feasible. |
| Key words: chondroitin sulfate egg shell membrane extraction response surface polysaccharide |
