| 摘要: |
| 建立了高效液相色谱测定啤酒瓶盖内衬垫中邻苯二甲酸二（2-乙基己基）酯（DEHP）含量的方法。通过改变样品的前处理方法及检测的色谱条件，以保留时间来定性，外标法来定量，对实验结果进行优化。实验得到:样品在经过无水乙醇浸泡处理，流动相甲醇与水的体积比为95∶5，紫外检测器检测波长为275 nm时，能获得的分离效果最佳;在该分析条件下，DEHP标准曲线呈现很好的线性关系，相关系数均大于0.998，精密度RSD<1.5%，DEHP在无水乙醇中浸泡的溶出量为24.45%，在95%甲醇中浸泡的溶出量为15.97%。 |
| 关键词: 高效液相色谱 邻苯二甲酸二（2-乙基己基）酯 啤酒瓶盖内衬垫 |
| DOI： |
| 分类号:TB487; TB484.3 |
| 基金项目:北京市教育委员会资助项目（KM201010015006） |
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| Determination of Diethylhexyl Phthalate in Inner Liner of Beer Bottle by High Performance Liquid Chromatography |
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FEI Fei, LI Ya-ling, ZHANG Kun, CHEN Qiang, LI Yu-ling
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Key Laboratory of Printing and Packaging Materials and Technology, Beijing Institute of Graphic Communication, Beijing 102600, China
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| Abstract: |
| A method of diethylhexyl phthalate quantitative analysis in inner liner of beer bottle by high performance liquid chromatography (HPLC) was established. The results were optimized by sample pre-treatment and the variation of chromatographic detection conditions. The retention time of peak was qualitatively employed to evaluate the results, and the ex-situ measurement was used to analyze quantitatively. When samples were extracted by soaking in ethanol solution, methanol: water = 95∶5 (volume /volume) was as mobile phase, and scanned with SPDM detector at 275 nm, the optimal separation phases was obtained. The results showed that standard curve of DEHP has good linearity with the correlation coefficient r2 higher than 0.998 and the precision was lower than 1.5%. It was confirmed that DEHP immigrates from the inner liner of beer bottle, the concentrations reach to 24.45% in ethanol solution, and 15.97% in 95% methanol solution. |
| Key words: HPLC diethylhexyl phthalate inner liner of beer bottle |
